Evaluation of Acute Oral Toxicity of Flavonoid Rutinoside on Liver Histopathology in Wistar Rats
Abstract
This study aimed to evaluate the acute oral toxicity of the flavonoid rutinoside on liver histopathology in Wistar rats, following OECD Guideline No. 423. Twelve Wistar rats were divided into four groups: male control and female control receiving oral administration of 5% dimethyl sulfoxide (DMSO), and treatment groups of male and female rats receiving rutinoside at a dose of 5000 mg/kg body weight (BW) in 5% DMSO orally. Post-treatment, liver histopathological examinations were performed to assess potential toxicological effects. The results indicated no signs of acute toxicity, as no significant histopathological alterations were observed in the liver tissues of the treated groups compared to the control groups. This study suggests that oral administration of rutinoside at 5000 mg/kg BW does not induce acute liver toxicity in Wistar rats under experimental settings.
Keywords: acute oral toxicity, liver, rutinoside
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Bassan, A., Alves, V. M., Amberg, A., Anger, L. T., Auerbach, S., Beilke, L., Bender, A., Cronin, M. T., Cross, K. P., Hsieh, J.-H., Greene, N., Kemper, R., Kim, M. T., Mumtaz, M., Noeske, T., Pavan, M., Pletz, J., Russo, D. P., Sabnis, Y., … Myatt, G. J. (2021). In silico approaches in organ toxicity hazard assessment: current status and future needs in predicting liver toxicity. Computational Toxicology, 20, 100187. https://doi.org/10.1016/j.comtox.2021.100187
Datta, S., Aggarwal, D., Sehrawat, N., Yadav, M., Sharma, V., Sharma, A., Zghair, A. N., Dhama, K., Sharma, A., Kumar, V., Sharma, A. K., & Wang, H. (2023). Hepatoprotective effects of natural drugs: Current trends, scope, relevance and future perspectives. Phytomedicine, 121, 155100. https://doi.org/10.1016/j.phymed.2023.155100
Dias, M. C., Pinto, D. C. G. A., & Silva, A. M. S. (2021). Plant Flavonoids: Chemical Characteristics and Biological Activity. Molecules, 26(17), 5377. https://doi.org/10.3390/molecules26175377
Elrasoul, A. S. A., Mousa, A. A., Orabi, S. H., Mohamed, M. A. E.-G., Gad-Allah, S. M., Almeer, R., Abdel-Daim, M. M., Khalifa, S. A. M., El-Seedi, H. R., & Eldaim, M. A. A. (2020). Antioxidant, Anti-Inflammatory, and Anti-Apoptotic Effects of Azolla pinnata Ethanolic Extract against Lead-Induced Hepatotoxicity in Rats. Antioxidants (Basel), 9(10), 1014. https://doi.org/10.3390/antiox9101014
Erhirhie, E. O., Ihekwereme, C. P., & Ilodigwe, E. E. (2018). Advances in acute toxicity testing: strengths, weaknesses and regulatory acceptance. Interdiscipline Toxicology, 11(1), 5–12. https://doi.org/10.2478/intox-2018-0001
Fritsche, K., Ziková-Kloas, A., Marx-Stoelting, P., & Braeuning, A. (2023). Metabolism-Disrupting Chemicals Affecting the Liver: Screening, Testing, and Molecular Pathway Identification. International Journal of Molecular Sciences, 24(3), 2686. https://doi.org/10.3390/ijms24032686
Ganeshpurkar, A., & Saluja, A. K. (2017). The Pharmacological Potential of Rutin. Saudi Pharm J, 25(2), 149–164. https://doi.org/10.1016/j.jsps.2016.04.025
Hasnat, H., Shompa, S. A., Islam, M. M., Alam, S., Richi, F. T., Emon, N. U., Ashrafi, S., Ahmed, N. U., Chowdhury, M. N. R., Fatema, N., Hossain, M. S., Ghosh, A., & Ahmed, F. (2024). Flavonoids: A treasure house of prospective pharmacological potentials. Heliyon, 10(6), e27533. https://doi.org/10.1016/j.heliyon.2024.e27533
Kale, V. P., Bebenek, I., Ghantous, H., Kapeghian, J., Singh, B. P., & Thomas, L. J. (2022). Practical Considerations in Determining Adversity and the No-Observed-Adverse-Effect-Level (NOAEL) in Nonclinical Safety Studies: Challenges, Perspectives and Case Studies. American College of Toxicology, 41(2). https://doi.org/10.1177/109158182110730
Krishnamurthy, P., Brown, M., Agrawal, S., & Short, R. F. (2017). Acute pancreatitis as a complication of trans-arterial chemoembolization of hepatocellular cancer—case report and review of literature. Journal of Gastrointestinal Oncology, 8(1), E26–E30. https://doi.org/10.21037/jgo.2017.01.15
Mah, E., Chen, O., Liska, D. J., & Blumberg, J. B. (2022). Dietary Supplements for Weight Management: A Narrative Review of Safety and Metabolic Health Benefits. Nutrients2, 14(9), 1787. https://doi.org/10.3390/nu14091787
Saeed, M., Munawar, M., Bi, J. B., Ahmed, S., Ahmad, M. Z., Kamboh, A. A., Arain, M. A., Naveed, M., & Chen, H. (2024). Promising phytopharmacology, nutritional potential, health benefits, and traditional usage of Tribulus terrestris L. herb. Heliyon, 10(4), e25549. https://doi.org/10.1016/j.heliyon.2024.e25549
Shayo, F., Sawe, H. R., Hyuha, G. M., Moshi, B., Gulamhussein, M. A., Mussa, R., Mdundo, W., Rwegoshora, S., Mfinanga, J. A., Kilindimo, S., & Weber, E. J. (2024). Clinical profile and outcomes of paediatric patients with acute seizures: a prospective cohort study at an urban emergency department of a tertiary hospital in Tanzania. Observational Study, 14(1), e069922. https://doi.org/10.1136/bmjopen-2022-069922
Suzuki, T., Morishita, T., Noda, T., & Ishiguro, K. (2015). Acute and Subacute Toxicity Studies on Rutin-Rich Tartary Buckwheat Dough in Experimental Animals. Journal of Nutritional Science and Vitaminology, 61(2), 175–181. https://doi.org/10.3177/jnsv.61.175
Tungmunnithum, D., Thongboonyou, A., Pholboon, A., & Yangsabai, A. (2018). Flavonoids and Other Phenolic Compounds from Medicinal Plants for Pharmaceutical and Medical Aspects: An Overview. Medicines, 5(3), 93. https://doi.org/doi: 10.3390/medicines5030093
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